Business and Management

All About Rabbit Antibody

Humans and mice have five antibody isotypes (IgA, IgD, IgE, IgG, and IgM). So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Interestingly, while humans have two IgA subclasses, mice have one IgA subclass, rabbits have a total of 13 IgA subclasses. On the other hand, rabbits have two κ light chain types K1 and K2.

For a more in-depth discussion of isotypes, subclasses, heavy and light chains, the composition of different isotypes in different species, and constant and variable regions. You can also get the best custom rabbit monoclonal antibodies services from various online sources.

Most of the antibodies for rabbit testing are of the IgG isotype. Rabbits have only one IgG subclass, while mice have IgG1, IgG2a, IgG2b, IgG2c, IgG3 subclasses, and mice have IgG1, IgG2a, IgG2b, IgG2c subclasses.

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Different isotypes and subclasses can have different molecular weights due to 1) the presence or absence of inter-chain disulfide bonds, 2) oligomerization of immunoglobulin molecules by intermolecular disulfide bonds, and 3) the number of immunoglobulin domains in the heavy chain. 

The common rabbit IgG isotype has a molecular weight of 150 kD, with two heavy chains (about 50 kD each) and two light chains (about 25 kD each) under non-reducing conditions. Under reduced conditions, the molecular weights were 50 and 25 kD for the heavy and light chains, respectively.

Antibodies produced in rabbits are essential for antigens that are non-immunogenic in rodents, which are the most common animal hosts for monoclonal antibody production.

Success has been achieved in transgenic rabbits with c-myc and v-abl transgenes for the initial production of a plasmacytoma cell line, and a rabbit monoclonal antibody has therefore been created.

Rabbit monoclonal antibodies can also be generated by phage display technology and alternative methods, especially since the rabbit hybridoma technology is patented. It has also been postulated that rabbit monoclonal antibodies are candidates for therapeutic agents against cancer, and rapid generation of rabbit monoclonal antibodies by massive systems has been tested.

Doug Parks